Mouse Cyp4a12a Polyclonal antibody, PBS Only

Cyp4a12a Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 51157-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse liver using 51157-1-AP (same clone as 51157-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 51157-1-AP (Cyp4a12a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51157-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 51157-1-AP (same clone as 51157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 51157-1-AP (Cyp4a12a antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 51157-1-AP (same clone as 51157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 51157-1-AP (Cyp4a12a antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51157-1-PBS in a different storage buffer formulation.

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Product Information

51157-1-PBS targets Cyp4a12a in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyp4a12a fusion protein Ag0509
• Full Name: cytochrome P450, family 4, subfamily a, polypeptide 12a
• Observed Molecular Weight: 58-70 kDa
• GenBank Accession Number: NM_177406
• Gene Symbol: Cyp4a12a
• Gene ID (NCBI): 277753
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. Mouse Cyp4a12a oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. For modification, the MW of Cyp4a12a is about 58-70 kDa in western blot detection.