Cyclin E2 Polyclonal antibody, PBS Only

Cyclin E2 Polyclonal Antibody for IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11935-1-PBS

Synonyms

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Validation Data Gallery

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  IP experiment of Jurkat using 11935-1-AP (same clone as 11935-1-PBS)

  IP result of anti-Cyclin E2 (IP:11935-1-AP, 4ug; Detection:11935-1-AP 1:300) with Jurkat cells lysate 4000ug. This data was developed using the same antibody clone with 11935-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11935-1-AP (same clone as 11935-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11935-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11935-1-AP (same clone as 11935-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11935-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 11935-1-AP (same clone as 11935-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11935-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 11935-1-AP (same clone as 11935-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11935-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11935-1-AP (same clone as 11935-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin E2 antibody (11935-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11935-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11935-1-AP (same clone as 11935-1-PBS)

  Immunofluorescent analysis of HeLa cells using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 11935-1-PBS in a different storage buffer formulation.

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Product Information

11935-1-PBS targets Cyclin E2 in IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin E2 fusion protein Ag2532
• Full Name: cyclin E2
• Calculated Molecular Weight: 374 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC020729
• Gene Symbol: CCNE2
• Gene ID (NCBI): 9134
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cyclin E2 (CCNE2) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of cyclindependent kinases (CDKs). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of cell cycle events. CCNE2 forms a complex with and functions as a regulatory subunit of CDK2 and has been shown to specifically interact with CIP/KIP family of CDK inhibitors. CCNE2 plays a role in cell cycle G1/S transition and its expression peaks at the G1-S phase. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells.