Cyclin B1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cyclin B1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat and More (5)

Applications

WB,IP,IHC,IF,ELISA

Conjugate

Unconjugated

Publications

60

Cat no : 28603-1-AP

Synonyms

CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1

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Validation Data Gallery

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  WB analysis using 28603-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28603-1-AP (Cyclin B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28603-1-AP

  WB result of Cyclin B1 antibody (28603-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells.

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  IP experiment of HeLa using 28603-1-AP

  IP result of anti-Cyclin B1 (IP:28603-1-AP, 4ug; Detection:28603-1-AP 1:2000) with HeLa cells lysate 1800 ug.

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  IHC staining of human skin cancer using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 28603-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cyclin B1 antibody (28603-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HT-29 using 28603-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Cyclin B1 antibody (28603-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 28603-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin B1 antibody (28603-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HT-29 cells, HeLa cells, K-562 cells, C6 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human skin cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 57 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

28603-1-AP targets Cyclin B1 in WB,IP,IHC,IF,ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Cited Reactivity: human, mouse, rat, chicken, canine, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin B1 fusion protein Ag29426
• Full Name: cyclin B1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC006510
• Gene Symbol: CCNB1
• Gene ID (NCBI): 891
• RRID: AB_2881179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins; Authors - Yulin Chen
human	WB	Clin Transl Med; Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3‐mediated N6‐methyladenosine modification of ICAM2 mRNA in fibroblast‐like synoviocytes; Authors - Jian Chen
human	WB	Cancer Lett; Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis.; Authors - Xintian Chen
human	WB	Mol Oncol; Interaction between membranous EBP50 and myosin 9 as a favorable prognostic factor in ovarian clear cell carcinoma; Authors - Mayu Nakagawa
human	WB	Life Sci; Periplocin inhibits hepatocellular carcinoma progression and reduces the recruitment of MDSCs through AKT/NF-κB pathway; Authors - Jia-Peng Lin
human,rat	WB,IHC	Am J Physiol Lung Cell Mol Physiol; KLF5 mediates vascular remodeling via HIF-1α in hypoxic pulmonary hypertension; Authors - Xiaochen Li