CoraLite®488 Plus TUNEL Assay Apoptosis Detection Kit

Excitation Laser

490 nm

Emission

515 nm

Application

FC, IF

Publications

25

Cat no : PF00006

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Validation Data Gallery

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  The above experimental results are based on Jurkat cells cultured for three days to perform a flow cytometry experiment to detect the apoptotic cells in the cell sample.

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  Staurosporine treated HeLa cells for 4.5 h.

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Product Information

A distinctive feature of apoptosis is degradation of DNA. The DNA fragments tend to be vary in length in integer multiples of 180 bp-200 bp, which is shown as a ladder-like pattern in agarose gel electrophoresis. This kit uses the TUNEL method, using Terminal Deoxynucleotidyl Transferase (TdT) to catalyze the incorporation of CL488-dUTP at the 3'-OH end of the broken DNA of apoptotic cells. CL488-dUTP-labeled DNA can be directly observed with a fluorescence microscope or quantified with a flow cytometer. The TUNEL method can selectively detect apoptotic cells, but not necrotic cells or cells with DNA strand breaks caused by irradiation and drug treatment.

Components

Components	20T	50T
CL488 TUNEL Reaction Buffer	1 mL	2 × 1.25 mL
TdT Enzyme	40 uL	100 uL
Proteinase K (2 mg/mL)	40 uL	100 uL
DNase I (2 U/μL）	5 uL	13 uL
10 X DNase I Buffer	100 uL	260 uL

Note: Due to the upgrade and optimization of some TUNEL series products, the original component "TUNEL Equilibration Buffer" has been removed from some products, which will not affect the effect of the assay, and the step of incubation with equilibration solution can be directly omitted from the TUNEL reaction procedure.

Storage

Store at -20°C. Avoid exposure to light. Stable for 2 years after shipment. Avoid freeze- thaw cycles.

Protocol

CoraLite® Plus 488 TUNEL Apoptosis Protocol

Conjugated

CoraLite® Plus 488 label, CL488 for short, this dye is similar to FITC and other dyes.

Ex/Em: 490/515nm

Cited in Article as

PF00006, CoraLite®488 Plus TUNEL Assay Apoptosis Detection Kit, Proteintech, IL, USA

Publications

Application	Title
	Pharmacol Res; Transfer of miR-877-3p via extracellular vesicles derived from dental pulp stem cells attenuates neuronal apoptosis and facilitates early neurological functional recovery after cerebral ischemia-reperfusion injury through the Bclaf1/p53 signaling pathway; Authors - Yan Miao
	Oxid Med Cell Longev; ProNGF/NGF Modulates Autophagy and Apoptosis through PI3K/Akt/mTOR and ERK Signaling Pathways following Cerebral Ischemia-Reperfusion in Rats.; Authors - Yanbo Li
	ACS Chem Neurosci; Tetrahydropalmatine Regulates BDNF through TrkB/CAM Interaction to Alleviate the Neurotoxicity Induced by Methamphetamine.; Authors - Lian Liu
	J Cell Physiol; Critical role of apoptosis in MeCP2-mediated epithelial-mesenchymal transition of ARPE-19 cells; Authors - Yongya Zhang
	ACS Biomater Sci Eng; siRNA Delivery with Stem Cell Membrane-Coated Magnetic Nanoparticles for Imaging-Guided Photothermal Therapy and Gene Therapy; Authors - Xupeng Mu
	J Cell Mol Med; The total xanthones extracted from Gentianella acuta alleviates HFpEF by activating the IRE1α/Xbp1s pathway; Authors - Linna Zhao