Cat no : PF00006
A distinctive feature of apoptosis is degradation of DNA. The DNA fragments tend to be vary in length in integer multiples of 180 bp-200 bp, which is shown as a ladder-like pattern in agarose gel electrophoresis. This kit uses the TUNEL method, using Terminal Deoxynucleotidyl Transferase (TdT) to catalyze the incorporation of CL488-dUTP at the 3'-OH end of the broken DNA of apoptotic cells. CL488-dUTP-labeled DNA can be directly observed with a fluorescence microscope or quantified with a flow cytometer. The TUNEL method can selectively detect apoptotic cells, but not necrotic cells or cells with DNA strand breaks caused by irradiation and drug treatment.
TUNEL Equilibration Buffer
2 X 1 mL
CL488 TUNEL Reaction Buffer
2 X 1.25 mL
Proteinase K (2 mg/mL)
DNase I (2 U/μL）
10 X DNase I Buffer
Store at -20°C. Avoid exposure to light. Stable for 2 years after shipment. Avoid freeze- thaw cycles.
CoraLite®488 label, CL488 for short, this dye is similar to FITC and other dyes.
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