Collagen Type XI Polyclonal antibody

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  WB analysis using 21841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 21841-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 21841-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 21841-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 21841-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 21841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Collagen Type XI antibody (21841-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Collagen Type XI Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 21841-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

COL11, COL11A1, CO11A1, COLL6, Collagen alpha 1(XI) chain

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Tested Applications

• Positive WB detected in: U2OS cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 5 publications below
• IF: See 4 publications below

Product Information

21841-1-AP targets Collagen Type XI in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16509

  Product name: Recombinant human COL11A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1429-1690 aa of BC117697

  Sequence: PLPILSSKKTRRHTEGMQADADDNILDYSDGMEEIFGSLNSLKQDIEHMKFPMGTQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTSGGETCIYPDKKSEGVRISSWPKEKPGSWFSEFKRGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYHCHQSAAWYDVSSGSYDKALRFLGSNDEEMSYDNNPFIKTLYDGCASRKGYEKTVIEINTPKIDQVPIVDVMINDFGDQNQKFGFEVGPVCFLG

• Full Name: collagen, type XI, alpha 1
• Calculated Molecular Weight: 1818 aa, 182 kDa
• Observed Molecular Weight: 250 kDa, 181 kDa
• GenBank Accession Number: BC117697
• Gene Symbol: COL11A1
• Gene ID (NCBI): 1301
• RRID: AB_2918074
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P12107
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COL11A1 is an important component of collagen XI and is considered to play an important role in a variety of connective tissue diseases. Type XI collagen is widely distributed in tissues including cartilage, bone, and muscle, and its hereditary absence in Marshall's syndrome, Stickler's syndrome, fibrochondrogenesis, and nonsyndromic hearing loss deafness, autosomal dominant 37 leads to facial and eye abnormalities, hearing loss, and joint problems. The COL11A1 expression was significantly correlated with the degree of immune infiltration and the expression of a variety of immune cell markers in tumor tissue. (PMID: 36160004 33610546)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Transl Oncol; Multiregion single cell analysis reveals a novel subtype of cancer-associated fibroblasts located in the hypoxic tumor microenvironment in colorectal cancer; Authors - Nanxin Zheng
human	IHC	J Orthop Res; Increased Wnt5a/ROR2 signaling is associated with chondrogenesis in meniscal degeneration; Authors - Yusuke Inoue
human	IHC,IF	BMC Med Genomics; Analysis of cancer-associated fibroblasts related genes identifies COL11A1 associated with lung adenocarcinoma prognosis; Authors - Haosheng Zheng
human	IF	Cancer Res; Dissecting FAP+ Cell Diversity in Pancreatic Cancer Uncovers an Interferon-Response Subtype of Cancer-Associated Fibroblasts with Tumor-Restraining Properties; Authors - Joshua Cumming
human	IHC	Oral Oncol; Single-cell transcriptomics reveal the effects of chemoimmunotherapy on hypopharyngeal cancer and its tumor microenvironment; Authors - Xiaoli Zhu
human	IHC,IF	Oncogene; Single-cell landscape of undifferentiated pleomorphic sarcoma; Authors - Yifei Lu