Collagen Type V Monoclonal antibody

Collagen Type V Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

CloneNo.

2C2C2

Cat no : 67604-1-Ig

Synonyms

COL5A1, Collagen alpha-1(V) chain, Collagen alpha 1(V) chain, 2C2C2

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Validation Data Gallery

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  WB analysis of HT-1080 using 67604-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67604-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67604-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67604-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67604-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 67604-1-Ig

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67604-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67604-1-Ig (Collagen Type V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67604-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67604-1-Ig (Collagen Type V antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 67604-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Collagen Type V antibody (67604-1-Ig, Clone: 2C2C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

• Positive WB detected in: HT-1080 cells, mouse colon tissue, HeLa cells, pig colon tissue, rat colon tissue, Saos-2 cells, SiHa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Product Information

67604-1-Ig targets Collagen Type V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Purified native protein corresponding to human collagen type V
• Full Name: collagen, type V, alpha 1
• Calculated Molecular Weight: 184 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: NM_000093
• Gene Symbol: COL5A1
• Gene ID (NCBI): 1289
• RRID: AB_2882810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Theranostics; Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.; Authors - Yinan Chen
human	IHC	Exp Ther Med; COL3A1, COL5A1 and COL6A2 serve as potential molecular biomarkers for osteoarthritis based on weighted gene co‑expression network analysis bioinformatics analysis; Authors - Yufeng Zhang
human	IHC	Neuropathology; Immunohistochemical expression of osteopontin and collagens in choroid plexus of human brains.; Authors - Keiji Wakamatsu
human	WB,IHC,IF	Comput Biol Med; Identification and targeting of cancer-associated fibroblast signature genes for prognosis and therapy in Cutaneous melanoma; Authors - Guokun Zhang
		J Adv Res; Mesenteric adipose-derived exosomal TINAGL1 enhances intestinal fibrosis in Crohn's Disease via SMAD4; Authors - Yidong Chen
human,mouse	WB	Cell Signal; Activated PARP1/FAK/COL5A1 signaling facilitates the tumorigenesis of cholesterol-resistant ovarian cancer cells through promoting EMT; Authors - Zeyin He