COL5A1 Polyclonal antibody

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• COL5A1 Polyclonal antibody (28382-1-AP)

COL5A1 Polyclonal Antibody for IHC, ELISA

Cat No. 28382-1-AP

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

Collagen alpha 1(V) chain, Collagen alpha-1(V) chain, Collagen Type V

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  IHC staining of human colon using 28382-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 28382-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 28382-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 28382-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 28382-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 28382-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: human colon tissue, human lung cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28382-1-AP targets COL5A1 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: collagen, type V, alpha 1
• Calculated Molecular Weight: 184 kDa
• GenBank Accession Number: NM_000093
• Gene Symbol: COL5A1
• Gene ID (NCBI): 1289
• RRID: AB_2881129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20908
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

5.0 out of 5

2 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Liliana (Verified Customer) (02-10-2024)

heat induced pretreatment EDTA, ph9

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: skin, keloid scar

Liliana (Verified Customer) (02-10-2024)

heat induced pre-treatment, EDTA, ph9 worked very well on keloid scar, normal skin, scleroderma, lichen slerosus

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: normal skin