Collagen Type V Polyclonal antibody

Collagen Type V Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, ELISA

Conjugate

Unconjugated

Reviews (2)

Cat no : 28382-1-AP

Synonyms

COL5A1, Collagen alpha 1(V) chain, Collagen Type V, collagen, type V, alpha 1

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Validation Data Gallery

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  IHC staining of human colon using 28382-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 28382-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28382-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28382-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 28382-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 28382-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28382-1-AP (Collagen Type V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human colon tissue, human lung cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28382-1-AP targets Collagen Type V in IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Purified native protein corresponding to human collagen type V
• Full Name: collagen, type V, alpha 1
• Calculated Molecular Weight: 184 kDa
• GenBank Accession Number: NM_000093
• Gene Symbol: COL5A1
• Gene ID (NCBI): 1289
• RRID: AB_2881129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Liliana (Verified Customer) (02-10-2024)

heat induced pretreatment EDTA, ph9

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: skin, keloid scar

FH

Liliana (Verified Customer) (02-10-2024)

heat induced pre-treatment, EDTA, ph9 worked very well on keloid scar, normal skin, scleroderma, lichen slerosus

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: normal skin