Claudin 2 Polyclonal antibody

Claudin 2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 26912-1-AP

Synonyms

CLDN2, SP82, PSEC0059



Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, Caco-2 cells, HT-29 cells, HepG2 cells, PANC-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26912-1-AP targets Claudin 2 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Claudin 2 fusion protein Ag25527
Full Name claudin 2
Calculated Molecular Weight 25 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC014424
Gene Symbol Claudin 2
Gene ID (NCBI) 9075
RRIDAB_2918115
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudin 2, also named as SP82, belongs to the claudin family. Claudin 2 is a tetraspan membrane protein consisting of two extracellular domains (ECL1 and ECL2), a small intracellular loop connecting the second and third transmembrane sections and short intracellular N and C terminal portions. Claudin 2 is a cation-selective channel forming TJ protein expressed in leaky epithelia. Claudin 2 is a 230 amino acid transmembrane protein, with a calculated molecular mass of 25 kDa (PMID: 31726679)

Protocols

Product Specific Protocols
WB protocol for Claudin 2 antibody 26912-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Ethnopharmacol

The rhizomes of Atractylodes macrocephala Koidz improve gastrointestinal health and pregnancy outcomes in pregnant mice via modulating intestinal barrier and water-fluid metabolism

Authors - Wang Chenxing
humanWB

Bioconjug Chem

Transforms of Cell Surface Glycoproteins Charge Influences Tumor Cell Metastasis via Atypically Inhibiting Epithelial-Mesenchymal Transition Including Matrix Metalloproteinases and Cell Junctions

Authors - Mingzhe Wang
mouseWB

Naunyn Schmiedebergs Arch Pharmacol

5-aminosalicylic acid alleviates colitis and protects intestinal barrier function by modulating gut microbiota in mice

Authors - Jingjing Wang
mouseIF

Front Nutr

Lactobacillus fermentum CECT5716 Alleviates the Inflammatory Response in Asthma by Regulating TLR2/TLR4 Expression

Authors - Weifang Wang
mouseIF

Diabetes Metab Syndr Obes

Effects of Different Carbohydrate Content Diet on Gut Microbiota and Aortic Calcification in Diabetic Mice

Authors - Xinyi Shen
mouseWB,IF

Front Pharmacol

Identification of claudin-2 as a promising biomarker for early diagnosis of pre-diabetes

Authors - Yang Songtao