Cas9 Polyclonal antibody

Cas9 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

bacteria and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26758-1-AP

Synonyms

Cas9, Cas9 7A9, CRISPR, CRISPR/Cas9, CRISPR-associated endonuclease Cas9, csn1, SpyCas9

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Validation Data Gallery

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  WB analysis of HeLa using 26758-1-AP

  HeLa cells transfected with CRISPR-Cas9 were subjected to SDS PAGE followed by western blot with 26758-1-AP (Cas9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 26758-1-AP

  A431 cells transfected with CRISPR-Cas9 were subjected to SDS PAGE followed by western blot with 26758-1-AP (Cas9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

26758-1-AP targets Cas9 in WB, IP, ELISA applications and shows reactivity with bacteria samples.

• Tested Reactivity: bacteria
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cas9 fusion protein Ag25281
• Full Name: Cas9
• Calculated Molecular Weight: 160 kDa
• GenBank Accession Number: NP_269215
• RRID: AB_2918109
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes. This antibody detects the sp Cas9.

Publications

Species	Application	Title
	IP	Nature; Enhancer release and retargeting activates disease-susceptibility genes.; Authors - Soohwan Oh
human	WB	Clin Epigenetics; E2F1 combined with LINC01004 super-enhancer to promote hepatocellular carcinoma cell proliferation and metastasis; Authors - Jingxuan Li