Tested Applications
| Positive WB detected in | human heart tissue, rat heart tissue, mouse heart tissue, pig heart tissue, rabbit heart tissue |
| Positive IHC detected in | human heart tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66376-1-Ig targets Cardiac Troponin I in WB, IHC, IF-P, ELISA, ISFET applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16758 Product name: Recombinant human TNNI3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-110 aa of BC096165 Sequence: MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEE Predict reactive species |
| Full Name | troponin I type 3 (cardiac) |
| Calculated Molecular Weight | 210 aa, 24 kDa |
| Observed Molecular Weight | 26-28 kDa |
| GenBank Accession Number | BC096165 |
| Gene Symbol | Cardiac Troponin I |
| Gene ID (NCBI) | 7137 |
| RRID | AB_2881753 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P19429 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Cardiac Troponin I antibody 66376-1-Ig | Download protocol |
| IHC protocol for Cardiac Troponin I antibody 66376-1-Ig | Download protocol |
| WB protocol for Cardiac Troponin I antibody 66376-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cardiac Troponin I Monoclonal antibody (1D5D6), catalog 66376-1-Ig, has 29 citations. These appear in journals including Bioactive Materials, ACS Nano, MedComm, Advanced Science, Biomaterials, Cardiovascular Research, Cell Death & Disease, Molecular Therapy, FASEB Journal, Journal of the American Heart Association, and International Journal of Molecular Medicine. Research fields center on cardiology—myocardial infarction, cardiomyopathy, heart regeneration, cardiotoxicity, and ferroptosis—using applications such as IF, WB, and IHC across mouse, rat, human, and pig.
| Species | Application | Title |
|---|---|---|
Bioact Mater Silicate biomaterials-based multicellular scaffolds with specific cellular spatial distribution for infarcted myocardium repair. | ||
ACS Nano Collagen-Targeting Self-Assembled Nanoprobes for Multimodal Molecular Imaging and Quantification of Myocardial Fibrosis in a Rat Model of Myocardial Infarction | ||
Adv Sci (Weinh) An Alternating Current Electroosmotic Flow-Based Ultrasensitive Electrochemiluminescence Microfluidic System for Ultrafast Monitoring, Detection of Proteins/miRNAs in Unprocessed Samples | ||
MedComm (2020) The ischemia-enhanced myocardial infarction protection-related lncRNA protects against acute myocardial infarction | ||
Biomaterials Localized delivery of anti-inflammatory agents using extracellular matrix-nanostructured lipid carriers hydrogel promotes cardiac repair post-myocardial infarction | ||
Cardiovasc Res Depleting inositol pyrophosphate 5-InsP7 protected the heart against ischaemia-reperfusion injury by elevating plasma adiponectin |
Reviews
What customers say
All three reviews rate this antibody 5 stars. Users report excellent performance across multiple applications: Western Blot in rat heart tissue, Immunohistochemistry and Immunofluorescence in human iPSC-derived cardiac cells and cardiac organoids, and Immunofluorescence in HL-1 cardiomyocytes. Recommended dilutions range from 1:100 to 1:1000 depending on the application. One user specifically noted successful overnight incubation with 3D PFA-fixed tissue at 1:500 and 2D PFA-fixed cells at 1:1000. Overall, reviewers consistently describe the antibody as working well with strong, reliable staining.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sarah-Eve (Verified Customer) (02-09-2026) | Works well for Western blot in rat right ventricle
 |
Refik (Verified Customer) (12-07-2025) | Works well with overnight incubation of human iPSC-derived cardiac cells and cardiac organoids using concentration 1:500-1000 for ICC of 5% PFA fixed and 0.1/1% triton-x permeabilized cells and tissues.
|
KARTHIKEYAN (Verified Customer) (11-20-2019) | We used this antibody to stain HL-1 cardiomyocyte cells, and the antibody works really well.
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