Calsequestrin 2 Polyclonal antibody

Calsequestrin 2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27560-1-AP

Synonyms

Calsequestrin 2, CASQ2, PDIB2

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Validation Data Gallery

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  WB analysis of mouse heart using 27560-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 27560-1-AP (Calsequestrin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 27560-1-AP

  IP result of anti-Calsequestrin 2 (IP:27560-1-AP, 4ug; Detection:27560-1-AP 1:1000) with mouse heart tissue lysate 3720 ug.

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  IHC staining of human heart using 27560-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27560-1-AP (Calsequestrin 2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 27560-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27560-1-AP (Calsequestrin 2 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skeletal muscle using 27560-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 27560-1-AP (Calsequestrin 2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 27560-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 27560-1-AP (Calsequestrin 2 antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27560-1-AP targets Calsequestrin 2 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calsequestrin 2 fusion protein Ag26412
• Full Name: calsequestrin 2 (cardiac muscle)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC022288
• Gene Symbol: Calsequestrin 2
• Gene ID (NCBI): 845
• RRID: AB_2880908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calsequestrin 2 (CASQ2) specifies the cardiac muscle family member of the calsequestrin family. Calsequestrin is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. CASQ2 is an important component of calcium handling as a calcium storage protein in the sarcoplasmic reticulum (SR), playing an important role in the regulation of the cardiac ryanodine receptor (RyR2)-mediated calcium release and SR calcium release refractoriness. Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.