Filter: AllWBIHCIF-FroIF/ICCFC (Intra)Affinity

WB analysis using 82811-1-RR (same clone as 82811-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 82811-1-RR (Calretinin antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 82811-1-RR (same clone as 82811-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82811-1-RR (Calretinin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 82811-1-RR (same clone as 82811-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82811-1-RR (Calretinin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 82811-1-RR (same clone as 82811-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82811-1-RR (Calretinin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.
IF Staining of adult mouse cerebellum tissue using 82811-1-RR (same clone as 82811-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using Calretinin antibody (82811-1-RR) at dilution of 1:500 and Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L). (Image produced by Watt Lab at McGill University) This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.
IF Staining of SH-SY5Y using 82811-1-RR (same clone as 82811-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Calretinin antibody (82811-1-RR, Clone: 2F20 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.
FC experiment of SH-SY5Y using 82811-1-RR (same clone as 82811-1-PBS)
1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Calretinin Recombinant antibody (82811-1-RR, Clone:2F20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.
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1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Calretinin Recombinant antibody (82811-1-RR, Clone:2F20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 82811-1-PBS
Biolayer interferometry (BLl) kinetic assay of 82811-1-PBS against Human Calretinin was performed. The affinity constant is 7.60 nM.

Calretinin Recombinant monoclonal antibody, PBS Only

Calretinin Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 82811-1-PBS

Clone No.2F20

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

CALB2, 29 kDa calbindin, 2F20, CAB29, CAL2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

82811-1-PBS targets Calretinin in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag2924 Product name: Recombinant human Calretinin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-271 aa of BC015484 Sequence: MAGPQQQPPYLHLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQHVGSSTEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMKLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMSLAEAGKLYRKDLEIVLCSEPPM Predict reactive species
Full Name	calbindin 2
Calculated Molecular Weight	29 kDa
Observed Molecular Weight	29 kDa
GenBank Accession Number	BC015484
Gene Symbol	Calretinin
Gene ID (NCBI)	794
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P22676
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.