Calponin Polyclonal antibody, PBS Only

Calponin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24855-1-PBS

Synonyms

CNN1, CNN 1, Calponin-1, Calponin 1, Basic calponin

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Validation Data Gallery

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  WB analysis of mouse stomach using 24855-1-AP (same clone as 24855-1-PBS)

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24855-1-AP (Calponin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 24855-1-AP (same clone as 24855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24855-1-AP (Calponin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 24855-1-AP (same clone as 24855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 24855-1-AP (same clone as 24855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 24855-1-AP (same clone as 24855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 24855-1-AP (same clone as 24855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 24855-1-AP (same clone as 24855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 24855-1-AP (same clone as 24855-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calponin antibody (24855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 24855-1-AP (same clone as 24855-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Calponin antibody (24855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24855-1-PBS in a different storage buffer formulation.

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Product Information

24855-1-PBS targets Calponin in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calponin fusion protein Ag21384
• Full Name: calponin 1, basic, smooth muscle
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC022015
• Gene Symbol: Calponin
• Gene ID (NCBI): 1264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kDa protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the specific region of human CNN1, thus it does not cross-react with CNN2 or CNN3.