Calcium Sensing Receptor Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Calcium Sensing Receptor Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19125-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 19125-1-AP (same clone as 19125-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19125-1-AP (Calcium Sensing Receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19125-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 19125-1-AP (same clone as 19125-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19125-1-AP (Calcium Sensing Receptor antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19125-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 19125-1-AP (same clone as 19125-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19125-1-AP (Calcium Sensing Receptor antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19125-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 19125-1-AP (same clone as 19125-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19125-1-AP (Calcium Sensing Receptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19125-1-PBS in a different storage buffer formulation.

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Product Information

19125-1-PBS targets Calcium Sensing Receptor in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: calcium-sensing receptor
• Calculated Molecular Weight: 121 kDa
• Observed Molecular Weight: 120-140 kDa
• GenBank Accession Number: NM_000388
• Gene Symbol: Calcium Sensing Receptor
• Gene ID (NCBI): 846
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CASR, also named as GPRC2A, PCAR1, belongs to the G-protein coupled receptor 3 family. Changes in extracellular calcium are thought to modulate a balance between proliferation and differentiation in a variety of cell types. The activity of CASR is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. It has been shown to play a major role in regulating parathyroid hormone secretion and subsequently influencing the calcium concentration of extracellular fluids. In normal primary keratinocytes and breast epithelial cells, proliferation is inhibited and elevated extracellular calcium levels trigger differentiation. Malignant transformations of these cell types are accompanied by a loss of responsiveness to the anti-proliferative effects of elevated extracellular calcium. Several disorders of calcium homeostasis have been linked to mutations in the CaSR. These include familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalemia (ADHypo). The 60-65 kDa band detected by this antibody might represent a degradation product of CASR (PMID: 15718278).