MT-CYB Polyclonal antibody

• 

  WB analysis using 55090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55090-1-AP (CYTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

MT-CYB Polyclonal Antibody for WB, ELISA

Cat No. 55090-1-AP

Publications(115)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, ELISA

Synonyms

CYTB, COB, Complex III subunit 3, Complex III subunit III, MT CYB

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 112 publications below
• IF: See 5 publications below

Product Information

55090-1-AP targets MT-CYB in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cytochrome b
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: YP_003024038
• Gene Symbol: CYTB
• Gene ID (NCBI): 4519
• RRID: AB_2881266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P00156
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYTB, also named as COB and MTCYB, is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. It's mitochondrion membrane protein with MW 43 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang
human	WB	Mol Cell; Hepatic micropeptide modulates mitochondrial RNA processing machinery in hepatocellular carcinoma; Authors - Linyu Zhu
human	WB	Nucleic Acids Res; Selection of initiator tRNA and start codon by mammalian mitochondrial initiation factor 3 in leaderless mRNA translation; Authors - Muhoon Lee
human	WB	Cell Rep Med; End binding-3 inhibitor activates regenerative program in age-related macular degeneration; Authors - Quinn Lee
human	WB	Nat Commun; RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.; Authors - Uwe Richter
mouse	WB	Nat Commun; Mitofusin 1 and 2 regulation of mitochondrial DNA content is a critical determinant of glucose homeostasis.; Authors - Vaibhav Sidarala

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Baptiste (Verified Customer) (05-23-2025)

Working well but have a light unspecific band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Lymphoblastoid

MT-CYB Antibody Western Blot validation (1:1000 dilution) in Lymphoblastoid (Cat no:55090-1-AP)

FH

Yan (Verified Customer) (10-21-2024)

Worked fine in WB assay. Perhaps I should've used more concentrated.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human primary fibroblasts

MT-CYB Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:55090-1-AP)

FH

Mi (Verified Customer) (02-10-2023)

Works very well in differentiated human white adipocyte cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: adipocytes