CYP4F12 Polyclonal antibody

CYP4F12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13243-1-AP

Synonyms

UNQ568/PRO1129, EC:1.14.14.1, Cytochrome P450 4F12, CYPIVF12

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Validation Data Gallery

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  WB analysis of human placenta using 13243-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13243-1-AP (CYP4F12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 13243-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13243-1-AP (CYP4F12 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13243-1-AP targets CYP4F12 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP4F12 fusion protein Ag3957
• Full Name: cytochrome P450, family 4, subfamily F, polypeptide 12
• Calculated Molecular Weight: 524 aa, 60 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC035350
• Gene Symbol: CYP4F12
• Gene ID (NCBI): 66002
• RRID: AB_2088412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP4F12(Cytochrome P450 4F12) Catalyzes leukotriene B4 omega-hydroxylation and arachidonic acid omega-hydroxylation but with an activity much lower than that of CYP4F2. It expresses a band of 60KDa in adult and fetal liver, kidney, placenta at term, seminal vesicles, and the prostate gland(PMID:15078342). And it can be phosphorylated in vivo(PMID:18828626).

Publications

Species	Application	Title
human	WB	Sci Rep; CYP4F12 is a potential biomarker and inhibits cell migration of head and neck squamous cell carcinoma via EMT pathway; Authors - Wenming Jia