CYP2D6/7 Polyclonal antibody

CYP2D6/7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 17868-1-AP

Synonyms

CPD6, CYP2D, CYP2D@, CYP2D6, CYP2D6/7, CYP2DL1, Cytochrome P450 2D6, P450 DB1, P450C2D, P450DB1

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Validation Data Gallery

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  WB analysis using 17868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17868-1-AP (CYP2D6/7 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17868-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17868-1-AP (CYP2D6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17868-1-AP (CYP2D6 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17868-1-AP (CYP2D6 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17868-1-AP (CYP2D6 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17868-1-AP (CYP2D6 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17868-1-AP (CYP2D6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17868-1-AP (CYP2D6 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17868-1-AP (CYP2D6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17868-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17868-1-AP (CYP2D6 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue, human liver tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:200-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 17868-1-AP is CYP2D6/7 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP2D6/7 fusion protein Ag12351
• Full Name: cytochrome P450, family 2, subfamily D, polypeptide 6
• Calculated Molecular Weight: 497 aa, 56 kDa
• Observed Molecular Weight: 48-56 kDa
• GenBank Accession Number: BC075023
• Gene Symbol: CYP2D6
• Gene ID (NCBI): 1565
• RRID: AB_2878457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP2D6(Cytochrome P450 2D6) is also named as CYP2DL1 and belongs to the cytochrome P450 family. It is a monooxygenase that involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. CYP2D6 is responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It has 2 isoforms produced by alternative splicing with the molecular weight of 56 kDa and 50 kDa. This antibody can recognize human CYP2D6/7, mouse CYP2D3/4/9/10/22/26, rat CYP2D1/3/4/10/26, due to the high homology.

Publications

Species	Application	Title
human	IHC	Theranostics; Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening.; Authors - Zhenyu Wang
human	WB	Drug Des Devel Ther; Effects of Saikosaponin D on CYP1A2 and CYP2D6 in HepaRG Cells.; Authors - Hongfang Li
human	WB	Drug Metab Pharmacokinet; Cloning, sequence analysis, and tissue expression of marmoset paraoxonase 1.; Authors - Shotaro Uehara
human,mouse	WB	Xenobiotica; Comparison of mouse and human cytochrome P450 mediated-drug metabolising activities in hepatic and extrahepatic microsomes.; Authors - Shotaro Uehara
rat	WB	Pharmazie; Silybin enhances the blood concentration of brexpiprazole in rats by downregulating expression of CYP3A4 and CYP2D6.; Authors - Congcong Yu
		J Adv Res; Gut microbiota and host Cyp450s co-contribute to pharmacokinetic variability in mice with non-alcoholic steatohepatitis: Effects vary from drug to drug; Authors - Jing Guo