Validation Data Gallery
|Positive WB detected in||human brain tissue|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10242-1-AP targets CYC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CYC1 fusion protein Ag0291|
|Full Name||cytochrome c-1|
|Calculated molecular weight||35 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC001006|
|Gene ID (NCBI)||1537|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CYC1, also named as Cytochrome c-1, belongs to the cytochrome c family. Cytochrome c1 is one respiratory subunit of the 11 subunits of the cytochrome bc1 complex of the mitochondrial electron-transfer chain. It mediates the transfer of an electron from Rieske iron-sulfur protein to cytochrome c(PMID:3036122 ). CYC1 mediates apoptosis. In this pathway, a variety of apoptotic stimuli cause CYC1 release from mitochondria, which in turn induces a series of biochemical reactions that result incaspase activation and subsequent cell death.(PMID:15189137) While CYC1 release and DNA fragmentation are unaffected by the noncleavablep75 mutant, mitochondrial morphology of dying cells is maintained, and loss of plasma membrane integrity is delayed(PMID:15186778). The protein migrated as a 35 kd band in SDS page.
Nat Cell Biol
PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.
Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV.
Caspase cleavage of cytochrome c1 disrupts mitochondrial function and enhances cytochrome c release.
Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.
Examination of bioenergetic function in the inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice.
Mitochondrial respiratory chain dysfunction mediated by ROS is a primary point of fluoride-induced damage in Hepa1-6 cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Daniel (Verified Customer) (09-16-2019)
The nitrocellulose membrane was blocked with PBS, 5% BSA, w/ Tween-20, yet high levels of background was observed.