Validation Data Gallery
|Positive WB detected in||Jurkat cells, human brain tissue, human liver tissue, mouse liver tissue, rat brain tissue, mouse brain tissue, L02 cells, rat liver tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, mouse cortex|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11733-1-AP targets CUX1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CUX1 fusion protein Ag2335|
|Full Name||cut-like homeobox 1|
|Calculated molecular weight||676 aa, 77 kDa|
|Observed molecular weight||80 kDa|
|GenBank accession number||BC025422|
|Gene ID (NCBI)||1523|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CUX1 is a transcription factors that regulates a large number of genes and microRNAs involved in multiple cellular processes, such as DNA replication, progression into S phase and later, the spindle assembly checkpoint that controls progression through mitosis. Cux1, a marker of supragranular layers. CUX1 acts by preventing binding of positively-acting CCAAT factors to promoters to mediated its gene reperssion. Also it's a component of nf-munr repressor, and binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. It can represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. CUX1 protein containing three cut repeats and a homeodomain called p200, there are several truncated isoforms that have been identified. These isoforms include a testis-specific isoform containing one cut repeat and the homeodomain called p55. In situ hybridization showed that mRNA for p55 was most abundant in round spermatids. Additional isoforms include p75, a protein similar in structure to p55, derived from the use of an alternate promoter in intron 20 ; the cut alternately spliced protein (CASP), a Golgi protein that contains amino-terminal sequences but none of the cut repeats or homeodomains ; and several other isoforms (p80, p90, p110, and p150) that appear to arise via proteolytic processing ]. This antibody can recognizes full-length p200 Cux1 and CASP protein (70~80kDa).
|Product Specific Protocols|
|WB protocol for CUX1 antibody 11733-1-AP||Download protocol|
|IHC protocol for CUX1 antibody 11733-1-AP||Download protocol|
|IF protocol for CUX1 antibody 11733-1-AP||Download protocol|
|IP protocol for CUX1 antibody 11733-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Reversing a model of Parkinson's disease with in situ converted nigral neurons.
Divergent transcriptional regulation of astrocyte reactivity across disorders.
Inactivating CUX1 mutations promote tumorigenesis.
IgSF11 homophilic adhesion proteins promote layer-specific synaptic assembly of the cortical interneuron subtype.
Cut-like homeobox 1 (CUX1) tumor suppressor gene haploinsufficiency induces apoptosis evasion to sustain myeloid leukemia.
Stem cell competition driven by the Axin2-p53 axis controls brain size during murine development
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (04-17-2023)
very good for WB
Etienne (Verified Customer) (12-19-2022)
Worked once with some noisy staining at 1/200. Didn't work at any other dilution
Carly (Verified Customer) (09-27-2019)
Used in an antibody array to identify proteins in human EDTA plasma.