CUX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CUX1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

66

Reviews (4)

Cat no : 11733-1-AP

Synonyms

CASP, CCAAT displacement protein, CDP, CDP/Cut, CDP1, Clox, COY1, cut like homeobox 1, CUTL1, CUX, Cux/CDP, CUX1, GOLIM6, Homeobox protein cut like 1, Homeobox protein cux 1, Nbla10317, p100, p110, p200, p75

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Validation Data Gallery

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  WB analysis using 11733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11733-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11733-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11733-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11733-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11733-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11733-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11733-1-AP

  IP result of anti-CUX1 (IP:11733-1-AP, 4ug; Detection:11733-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 11733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cortex using 11733-1-AP

  IF result of the antibody CUX1 (11733-1-AP, 1:300) with mouse cortex, Data from Dr. Lee O. Vaasjo Muñoz, University of Chicago. .

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Tested Applications

• Positive WB detected in: Jurkat cells, human brain tissue, human liver tissue, mouse liver tissue, rat brain tissue, mouse brain tissue, L02 cells, rat liver tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse cortex

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 14 publications below
• IHC: See 18 publications below
• IF: See 34 publications below
• IP: See 1 publications below

Product Information

11733-1-AP targets CUX1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CUX1 fusion protein Ag2335
• Full Name: cut-like homeobox 1
• Calculated Molecular Weight: 676 aa, 77 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC025422
• Gene Symbol: CUX1
• Gene ID (NCBI): 1523
• RRID: AB_2086995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CUX1 is a transcription factors that regulates a large number of genes and microRNAs involved in multiple cellular processes, such as DNA replication, progression into S phase and later, the spindle assembly checkpoint that controls progression through mitosis. Cux1, a marker of supragranular layers. CUX1 acts by preventing binding of positively-acting CCAAT factors to promoters to mediated its gene reperssion. Also it's a component of nf-munr repressor, and binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. It can represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. CUX1 protein containing three cut repeats and a homeodomain called p200, there are several truncated isoforms that have been identified. These isoforms include a testis-specific isoform containing one cut repeat and the homeodomain called p55. In situ hybridization showed that mRNA for p55 was most abundant in round spermatids. Additional isoforms include p75, a protein similar in structure to p55, derived from the use of an alternate promoter in intron 20 ; the cut alternately spliced protein (CASP), a Golgi protein that contains amino-terminal sequences but none of the cut repeats or homeodomains ; and several other isoforms (p80, p90, p110, and p150) that appear to arise via proteolytic processing ]. This antibody can recognizes full-length p200 Cux1 and CASP protein (70~80kDa).

Publications

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	Nat Genet; Inactivating CUX1 mutations promote tumorigenesis.; Authors - Chi C Wong ; KD Validated
mouse	IF	Sci Adv; IgSF11 homophilic adhesion proteins promote layer-specific synaptic assembly of the cortical interneuron subtype.; Authors - Yasufumi Hayano
human,mouse	WB	Nat Commun; Cut-like homeobox 1 (CUX1) tumor suppressor gene haploinsufficiency induces apoptosis evasion to sustain myeloid leukemia.; Authors - Emmanuelle Supper ; KD Validated
mouse	IHC	Dev Cell; Stem cell competition driven by the Axin2-p53 axis controls brain size during murine development; Authors - Xue-Lian Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (04-22-2024)

I tested Cux1 in human brain FFPE tissue and it stained nicely, although the layers are not really sharp

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

CUX1 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:11733-1-AP)

FH

Boyan (Verified Customer) (04-17-2023)

very good for WB

• Applications: Western Blot

FH

Etienne (Verified Customer) (12-19-2022)

Worked once with some noisy staining at 1/200. Didn't work at any other dilution

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/10,1/20,1/100,1/200
• Cell Tissue Type: Human Brain Organoids

CUX1 Antibody Immunofluorescence validation (1/10,1/20,1/100,1/200 dilution) in Human Brain Organoids (Cat no:11733-1-AP)

FH

Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

• Applications: Other,
• Cell Tissue Type: Human EDTA Plasma