CUTC Polyclonal antibody

CUTC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15030-1-AP

Synonyms

CGI-32

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Validation Data Gallery

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  WB analysis of HeLa using 15030-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15030-1-AP (CUTC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15030-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15030-1-AP (CUTC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human endometrial cancer using 15030-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15030-1-AP (CUTC antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 15030-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15030-1-AP (CUTC antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas using 15030-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15030-1-AP (CUTC antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 15030-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15030-1-AP (CUTC antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15030-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUTC antibody (15030-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse skeletal muscle tissue
• Positive IHC detected in: human endometrial cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15030-1-AP targets CUTC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CUTC fusion protein Ag2126
• Full Name: cutC copper transporter homolog (E. coli)
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC021105
• Gene Symbol: CUTC
• Gene ID (NCBI): 51076
• RRID: AB_2086984
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.