CTTNBP2NL Polyclonal antibody

CTTNBP2NL Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25523-1-AP

Synonyms

KIAA1433, CTTNBP2 N-terminal-like protein, CTTNBP2 N terminal like

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Validation Data Gallery

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  WB analysis using 25523-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25523-1-AP (CTTNBP2NL antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 25523-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 25523-1-AP (CTTNBP2NL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 25523-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25523-1-AP (CTTNBP2NL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 25523-1-AP

  IP result of anti-CTTNBP2NL (IP:25523-1-AP, 4ug; Detection:25523-1-AP 1:600) with MCF-7 cells lysate 3200ug.

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  IHC staining of human ovary cancer using 25523-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25523-1-AP (CTTNBP2NL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 25523-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CTTNBP2NL antibody (25523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of U2OS using 25523-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CTTNBP2NL antibody (25523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, MDA-MB-453s cells, mouse skin tissue, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

25523-1-AP targets CTTNBP2NL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTTNBP2NL fusion protein Ag22137
• Full Name: CTTNBP2 N-terminal like
• Calculated Molecular Weight: 639 aa, 70 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC016029
• Gene Symbol: CTTNBP2NL
• Gene ID (NCBI): 55917
• RRID: AB_2880117
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	PLoS Pathog; Defining the proteolytic landscape during enterovirus infection.; Authors - Mohsan Saeed