Cortactin Polyclonal antibody

Cortactin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (1)

Cat no : 11381-1-AP

Synonyms

CTTN, SRC8, EMS1

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Validation Data Gallery

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  WB analysis using 11381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 11381-1-AP

  IP result of anti-Cortactin (IP:11381-1-AP, 4ug; Detection:11381-1-AP 1:1000) with MCF-7 cells lysate 2400 ug.

• 

  IHC staining of human breast cancer using 11381-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11381-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11381-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11381-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11381-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cortactin antibody (11381-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, HUVEC cells, HT-1080 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Product Information

11381-1-AP targets Cortactin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cortactin fusion protein Ag1940
• Full Name: cortactin
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 70-85 kDa
• GenBank Accession Number: BC008799
• Gene Symbol: Cortactin
• Gene ID (NCBI): 2017
• RRID: AB_2088125
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).

Publications

Species	Application	Title
mouse	IF	Circ. Res.; Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.; Authors - Dong Ma
	WB	Genes Dev; Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein; Authors - Megan L Norris
human	IF	Adv Healthc Mater; Fiber Microarchitecture in Interpenetrating Collagen-Alginate Hydrogel with Tunable Mechanical Plasticity Regulates Tumor Cell Migration; Authors - Zhao Wei
human	WB	EMBO J; TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.; Authors - Qichao Huang
human	IHC	Front Oncol; A four oxidative stress gene prognostic model and integrated immunity-analysis in pancreatic adenocarcinoma; Authors - Hao Wang
rat	WB	Neuroreport; Decreased expression of cortactin in the schizophrenia brain.; Authors - Hriday P Bhambhvani

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angie (Verified Customer) (03-11-2024)

The antibody detected a band around 80 kD at dilution of 1:5000 incubated at room temperature for 2 hour followed by secondary antibody donkey-anti-rabbit (Alexa Fluor 800) at 1:20000 dilution, incubated 1 hour at room temperature.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: A549, HEK293, SHSY-5Y

Cortactin Antibody Western Blot validation (1:5000 dilution) in A549, HEK293, SHSY-5Y (Cat no:11381-1-AP)

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