CTNNBIP1 Recombinant antibody, PBS Only (Detector)

CTNNBIP1 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230164B3

Cat no : 82914-2-PBS

Synonyms

Inhibitor of beta-catenin and Tcf-4, ICAT, Beta-catenin-interacting protein 1, 230164B3

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00102-1

  Sandwich ELISA standard curve of MP00102-1, Human CTNNBIP1 Recombinant Matched Antibody Pair - PBS only. 82914-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag13160. 82914-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

• 

  Sample test of MP00102-1

  Hela and HepG2 cell lysates were measured. The human CTNNBIP1 concentration of detected samples was determined to be 1,205.0 pg/mL (based on a 1.50 mg/mL extract load) in Hela cell lysate and 1486.5 pg/mL (based on a 1.20 mg/mL extract load) in HepG2 cell lysate.

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  Cytometric bead array standard curve of MP00102-1

  Cytometric bead array standard curve of MP00102-1, CTNNBIP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82914-4-PBS. Detection antibody: 82914-2-PBS. Standard: Ag13160. Range: 0.313-20 ng/mL.

• 

  Cytometric bead array standard curve of MP00102-3

  Cytometric bead array standard curve of MP00102-3, CTNNBIP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82914-3-PBS. Detection antibody: 82914-2-PBS. Standard: Ag13160. Range: 1.25-20 ng/mL.

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  Affinity and Kinetic Characterization of 82914-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 82914-2-PBS against Human CTNNBIP1 was performed. The affinity constant is 11.0 nM.

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Product Information

82914-2-PBS targets CTNNBIP1 as part of a matched antibody pair:

MP00102-1: 82914-4-PBS capture and 82914-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00102-3: 82914-3-PBS capture and 82914-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CTNNBIP1 fusion protein Ag13160
• Full Name: catenin, beta interacting protein 1
• Calculated Molecular Weight: 21 kDa
• GenBank Accession Number: BC014300
• Gene Symbol: CTNNBIP1
• Gene ID (NCBI): 56998
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.