CSF2RA/CD116 Polyclonal antibody

• Featured Product
• KD/KO Validated

CSF2RA/CD116 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18307-1-AP

Synonyms

CD116, GMCSFR alpha, GMCSFR, GM CSF R alpha, CSF2RA

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Validation Data Gallery

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  WB analysis of THP-1 using 18307-1-AP

  WB result of CSF2RA/CD116 antibody (18307-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSF2RA/CD116 transfected THP-1 cells.

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  WB analysis using 18307-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18307-1-AP (CSF2RA/CD116 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 18307-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18307-1-AP (CSF2RA/CD116 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 18307-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18307-1-AP (CSF2RA/CD116 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells, THP-1 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18307-1-AP targets CSF2RA/CD116 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC071835
• Gene Symbol: CD116
• Gene ID (NCBI): 1438
• RRID: AB_2229990
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD116, also named as CSF2RA CDw116 CSF2RAX CSF2RAY CSF2RX CSF2RY GM-CSF-R-alpha GMCSFR and GMR, is a low affinity receptor for granulocyte-macrophage colony-stimulating factor. CD116 transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.