CSF1R/CD115 Recombinant monoclonal antibody

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  WB analysis using 84477-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84477-6-RR (CSF1R antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 84477-6-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CSF1R antibody (84477-6-RR, Clone: 241675D4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84477-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84477-6-RR against Human CSF1R were performed. The affinity constant is 0.775 nM.

CSF1R/CD115 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84477-6-RR

Clone No.241675D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CSF1R, CSF-1 receptor, CSF 1 R, CD115, C FMS

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Tested Applications

• Positive WB detected in: THP-1 cells
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84477-6-RR targets CSF1R/CD115 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: colony stimulating factor 1 receptor
• Calculated Molecular Weight: 972 aa, 108 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC047521
• Gene Symbol: CSF1R/CD115
• Gene ID (NCBI): 1436
• RRID: AB_3671998
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P07333
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).