CRYBA2 Monoclonal antibody

CRYBA2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G1A9

Cat no : 68354-1-Ig

Synonyms

Beta A2 crystallin, Beta crystallin A2, CRYBA2, crystallin, beta A2

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Validation Data Gallery

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  WB analysis of rat eye using 68354-1-Ig

  rat eye tissue were subjected to SDS PAGE followed by western blot with 68354-1-Ig (CRYBA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 68354-1-Ig

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 68354-1-Ig (CRYBA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rat eye tissue, mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68354-1-Ig targets CRYBA2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CRYBA2 fusion protein Ag8244
• Full Name: crystallin, beta A2
• Calculated Molecular Weight: 197 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC006285
• Gene Symbol: CRYBA2
• Gene ID (NCBI): 1412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRYBA2, also named as Beta A2 crystallin, is a member of crystallin superfamily. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also defined as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Crystallins are the dominant structural components of the vertebrate eye lens. The MW of this protein is 22 kDa, and this antibody specially recognises the 22 kDa protein.