CPT1A Monoclonal antibody, PBS Only (Capture)

• Featured Product
• KD/KO Validated

CPT1A Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5D8G9

Cat no : 66039-1-PBS

Synonyms

CPT-1A, CPT1, CPT I, Carnitine O-palmitoyltransferase I, liver isoform, Carnitine O-palmitoyltransferase 1, liver isoform

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Validation Data Gallery

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  WB analysis of A549 using 66039-1-Ig (same clone as 66039-1-PBS)

  WB result of CPT1A antibody (66039-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPT1A transfected A549 cells. This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 66039-1-Ig (same clone as 66039-1-PBS)

  IP result of anti-CPT1A (IP:66039-1-Ig, 4ug; Detection:66039-1-Ig 1:500) with MCF-7 cells lysate 1200ug. This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66039-1-Ig (same clone as 66039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66039-1-Ig (same clone as 66039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66039-1-Ig (same clone as 66039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66039-1-Ig(CPT1A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66039-1-Ig (same clone as 66039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66039-1-Ig(CPT1A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66039-1-Ig (same clone as 66039-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CPT1A antibody (66039-1-Ig, Clone: 5D8G9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66039-1-Ig (same clone as 66039-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CPT1A Monoclonal antibody (66039-1-Ig, Clone:5D8G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50197-1

  Cytometric bead array standard curve of MP50197-1, CPT1A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66039-1-PBS. Detection antibody: 66039-2-PBS. Standard:Ag7745. Range: 0.195-50 ng/mL.

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Product Information

66039-1-PBS targets CPT1A as part of a matched antibody pair:

MP50197-1: 66039-1-PBS capture and 66039-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CPT1A fusion protein Ag7745
• Full Name: carnitine palmitoyltransferase 1A (liver)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC000185
• Gene Symbol: CPT1A
• Gene ID (NCBI): 1374
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.