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CPT1A Polyclonal antibody
CPT1A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 15184-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, HepG2 cells, A549 cells, mouse liver tissue, rat liver tissue, MCF-7 cells, U-251 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human liver cancer tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15184-1-AP targets CPT1A in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, yellow croaker, zebrafish, pig, duck, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CPT1A fusion protein Ag7202|
|Full Name||carnitine palmitoyltransferase 1A (liver)|
|Calculated molecular weight||88 kDa|
|Observed molecular weight||86 kDa|
|GenBank accession number||BC000185|
|Gene ID (NCBI)||1374|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. It is Localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.
|Product Specific Protocols|
|WB protocol for CPT1A antibody 15184-1-AP||Download protocol|
|IHC protocol for CPT1A antibody 15184-1-AP||Download protocol|
|IF protocol for CPT1A antibody 15184-1-AP||Download protocol|
|IP protocol for CPT1A antibody 15184-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (05-17-2021)
Antibody used at 1/1000 for 24 hours at 4 degrees. Non-specific bands were also present upon imaging.
Linlin (Verified Customer) (05-08-2019)
This CPT1A antibody is ideal for precipitation of CPT1A in cell lysates and immunoblot analysis of CPT1A. Recommend.