COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12375-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 12375-1-AP (same clone as 12375-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12375-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12375-1-AP (same clone as 12375-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12375-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12375-1-AP (same clone as 12375-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12375-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12375-1-AP (same clone as 12375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12375-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12375-1-AP (same clone as 12375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12375-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 12375-1-AP (same clone as 12375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12375-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12375-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12375-1-AP (same clone as 12375-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COX2/ Cyclooxygenase 2/ PTGS2 antibody (12375-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12375-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 12375-1-AP (same clone as 12375-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using COX2/ Cyclooxygenase 2/ PTGS2 antibody (12375-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12375-1-PBS in a different storage buffer formulation.

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Product Information

12375-1-PBS targets COX2/ Cyclooxygenase 2/ PTGS2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag3025
• Full Name: prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
• Calculated Molecular Weight: 604 aa, 68 kDa
• Observed Molecular Weight: 70-74 kDa
• GenBank Accession Number: BC013734
• Gene Symbol: COX2/PTGS2
• Gene ID (NCBI): 5743
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

COX2 is an enzyme involved in the synthesis of prostaglandins from arachidonic acid.

1. What is the molecular weight of COX2?

The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also 

expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented 

PTGS2 has also previously been detected in AD brains (PMID:14724276).

2. Is COX2 post-translationally modified?

COX2 is a subject of post-translational modifications, including phosphorylation, glycosylation, and 

s-nitrosylation (PMID: 28939645).

3. What is the difference between COX1, COX2, and COX3?

There are three isoenzymes that have cyclooxygenase activity: COX1, COX2, and COX3. While COX1 

is a ubiquitously expressed constitutive enzyme, expression of COX2 is generally low but can be rapidly 

induced by various stimuli (as part of infection and inflammatory response) and is controlled by the 

transcription factor NFκB. COX3 is an alternative splice variant of COX1 enzyme and is considered 

non-functional in humans. 

4. I cannot detect COX2 in my sample during western blotting.

Unlike COX1, COX2 expression is inducible by a variety of stimuli including certain growth factors, cytokines, 

and proinflammatory stimuli. COX2 basal expression levels may be very low in unstimulated cells. Additionally, 

the COX2 half-life time is short and after stimulation, COX2 protein can be quickly degraded to basal levels 

(PMID: 10966456), which should be taken into account during experimental design.

5. What is the role of COX2 in cancer?

COX2 is often upregulated in various cancer types and increased expression of COX2 is associated with 

greater angiogenesis of solid tumors, increased invasion, and metastasis, as well as decreased host immunity.

6. What is subcellular localization of COX2?

Both COX1 and COX2 are present in the endoplasmic reticulum (ER) and nuclear envelope (PMID: 9545330), 

but COX2 has been reported to be more enriched in the nuclear envelope compared to COX1 (PMID: 7738031).