Tested Applications
| Positive WB detected in | A431 cells, 143B cells, various samples, HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55070-1-AP targets MTCO2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, monkey, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | cytochrome c oxidase II |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 23-26 kDa |
| GenBank Accession Number | YP_003024029 |
| Gene Symbol | MTCO2 |
| Gene ID (NCBI) | 4513 |
| RRID | AB_10859832 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00403 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D). The antibody is specific to MTCO2.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for MTCO2 antibody 55070-1-AP | Download protocol |
| IF protocol for MTCO2 antibody 55070-1-AP | Download protocol |
| IHC protocol for MTCO2 antibody 55070-1-AP | Download protocol |
| IP protocol for MTCO2 antibody 55070-1-AP | Download protocol |
| WB protocol for MTCO2 antibody 55070-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MTCO2 (COX2) polyclonal antibody (Cat# 55070-1-AP) has accumulated 215 citations. Publications appear in high-impact journals including Cell, Nature Communications, Nature Metabolism, Molecular Cell, Cell Metabolism, Autophagy, Nature Microbiology, and Nucleic Acids Research, among over 80 journals. Research fields span mitochondrial biology and metabolism, cancer, neurodegeneration, autophagy/mitophagy, cardiovascular disease, inflammation, and metabolic disorders such as diabetes and obesity.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Fructose directly remodels the translocase of the outer membrane to impair oxidative phosphorylation in podocytes | ||
Cell Dynamic mapping of proteome trafficking within and between living cells by TransitID | ||
Cell Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis. | ||
Cell PAX translocations remodel mitochondrial metabolism through altered leucine usage in rhabdomyosarcoma | ||
Cell Metab Transketolase promotes MAFLD by limiting inosine-induced mitochondrial activity |
Reviews
What customers say
Most reviewers (8 of 9) rate this antibody 4–5 stars for Western Blot, typically at 1:1000 dilution. Users consistently report clear, sharp bands at the expected molecular weight with minimal nonspecific background across cell lines including HCT116, HEK293, fibroblasts, and K562. Performance is good on PVDF membranes with both ECL and fluorescent detection. One reviewer (2 stars) found it ineffective on zebrafish tissue for both WB and IF despite listed reactivity, requesting an alternative protocol. Overall, the antibody is well-regarded for human samples but may require optimization for non-human species.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Morgane (Verified Customer) (10-15-2025) | Works best on PVDF, very clear in total protein extract
 |
Matthieu (Verified Customer) (09-24-2025) | Band is clearly visible at the correct size
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Manon (Verified Customer) (09-24-2025) | vey nice band
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Emilie (Verified Customer) (09-24-2025) | Bands appeared sharp and well defined under the chosen dilution.
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Vignesh (Verified Customer) (09-03-2025) | It's a good product with reduced or no nonspecific bands.
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Lenie (Verified Customer) (07-18-2025) | The IF staining and WB with the followed protocol of ProteinTech did not work on zebrafish tissue, even it describes on the site it should. Is there a possibility to send a working protocol about this Abies for zebrafish? Thanks. The bond which shows is a human patient sample.
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Baptiste (Verified Customer) (05-23-2025) | Working perfectly
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Edward (Verified Customer) (11-15-2022) | Used with 12 ug protein lysate MTCO2 works quite well, both with ECL detection and fluorescent secondary antibodies. Sometimes a bit fainter with ECL.
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Maria (Verified Customer) (03-11-2021) | MTCO2 in treated Human Primary Fibroblasts. 10 ug of total protein. 12% gel. 1:1000 primary ab in BSA 3% in PBST O/N 4ºC incubation. Secondary ab HRP 1:5000 1h RT.
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