SARS-CoV-2 S protein (RBD, 319-541 aa) Monoclonal antibody

SARS-CoV-2 S protein (RBD, 319-541 aa) Monoclonal Antibody for WB, ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Virus and More (2)

Applications

WB, Neutralization, ELISA

Conjugate

Unconjugated

CloneNo.

1H3E9

Cat no : 67758-1-Ig

Synonyms

COVID-19 RBD, RBD, SARS-CoV-2 Spike Glycoprotein (319-541 aa), spike protein



Tested Applications

Positive WB detected inAg30688
Positive Neutralization detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
NEUTRALIZATIONNEUTRALIZATION : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 8 publications below

Product Information

67758-1-Ig targets SARS-CoV-2 S protein (RBD, 319-541 aa) in WB, Neutralization, ELISA applications and shows reactivity with Virus samples.

Tested Reactivity Virus
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SARS-CoV-2 S protein (RBD, 319-541 aa) fusion protein Ag30688
Full Name SARS-CoV-2 Spike Protein
Calculated Molecular Weight 141 kDa
GenBank Accession NumberNC_045512
Gene Symbol SARS-COV-2
Gene ID (NCBI) 43740568
RRIDAB_2918526
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616).

Lyophilized format of this product is available.

Protocols

Product Specific Protocols
WB protocol for SARS-CoV-2 S protein (RBD, 319-541 aa) antibody 67758-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Acta Pharm Sin B

A novel heterologous receptor-binding domain dodecamer universal mRNA vaccine against SARS-CoV-2 variants

Authors - Shugang Qin

J Med Virol

A novel three-plasmid packaging system for chimeric SFV/SIN VRPs derived from Semliki Forest virus and Sindbis virus as a candidate gene delivery vector

Authors - Yonghui Huang
humanWB

Biochem Pharmacol

Harringtonine: A more effective antagonist for Omicron variant

Authors - Shiling Hu

bioRxiv

Novel sensor-integrated proteome on chip (SPOC) platform with thousands of folded proteins on a 1.5 sq-cm biosensor chip to enable high-throughput real-time label-free screening for kinetic analysis

Authors - Chidozie Victor Agu
humanWB

Virol J

LINC01002 functions as a ceRNA to regulate FRMD8 by sponging miR-4324 for the development of COVID-19

Authors - Xinyi Kong
mouseWB

Int J Mol Sci

SARS-CoV-2 Rapidly Infects Peripheral Sensory and Autonomic Neurons, Contributing to Central Nervous System Neuroinvasion before Viremia

Authors - Jonathan D Joyce