COPS3 Recombinant antibody

COPS3 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

240918E3

Cat no : 83822-2-RR

Synonyms

JAB1-containing signalosome subunit 3, COP 9 signalosome complex subunit 3, 240918E3

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Validation Data Gallery

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  WB analysis using 83822-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83822-2-RR (COPS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 83822-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83822-2-RR (COPS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 83822-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using COPS3 antibody (83822-2-RR, Clone: 240918E3 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 83822-2-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using COPS3 antibody (83822-2-RR, Clone: 240918E3 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 83822-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COPS3 antibody (83822-2-RR, Clone: 240918E3 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 83822-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83822-2-RR against Human COPS3 were performed. The affinity constant is 1.29 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, HT-29 cells, HEK-293T cells, mouse ovary tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue
• Positive IF/ICC detected in: A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83822-2-RR is COPS3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: COPS3 fusion protein Ag32825
• Full Name: COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC001891
• Gene Symbol: COPS3
• Gene ID (NCBI): 8533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPS3 possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17.