COPB2 Polyclonal antibody

COPB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14733-1-AP

Synonyms

p102, Coatomer subunit beta', Beta'-COP, Beta'-coat protein

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Validation Data Gallery

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  WB analysis using 14733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14733-1-AP (COPB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 14733-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14733-1-AP (COPB2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14733-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14733-1-AP (COPB2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-12 using 14733-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using COPB2 antibody (14733-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 14733-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using COPB2 antibody (14733-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human stomach tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-12 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:600-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14733-1-AP targets COPB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COPB2 fusion protein Ag6498
• Full Name: coatomer protein complex, subunit beta 2 (beta prime)
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC000326
• Gene Symbol: COPB2
• Gene ID (NCBI): 9276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.