Collagen Type XXVII Polyclonal antibody

Collagen Type XXVII Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15673-1-AP

Synonyms

COL27A1, Collagen alpha 1(XXVII) chain, Collagen Type XXVII, collagen, type XXVII, alpha 1, KIAA1870

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Validation Data Gallery

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  WB analysis using 15673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15673-1-AP (Collagen Type XXVII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 15673-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15673-1-AP (Collagen Type XXVII antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse brain tissue, mouse heart tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15673-1-AP targets Collagen Type XXVII in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Collagen Type XXVII fusion protein Ag8140
• Full Name: collagen, type XXVII, alpha 1
• Calculated Molecular Weight: 1860 aa, 87 kDa
• Observed Molecular Weight: 68-73 kDa
• GenBank Accession Number: BC007696
• Gene Symbol: COL27A1
• Gene ID (NCBI): 85301
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Collagen Type XXVII, also named COL27A1, it is a member of the collagen fibrillar gene family . It is located in the extrcellular matrix. The calculated molecular weight of COL27A1 is 186 kDa. This antibody can recognize the 73 kDa isoform of target. The high level of Col27a1 expression in mouse cartilage suggested the possibility that Col27a1 may be regulated in cartilage by SOX9, as are Col2a1, Col11a2, Col9a1, and Col9a2 (PMID: 15922909). Expression analyses of the mouse col27a1 gene demonstrated high expression in cartilage, the eye and ear, but also in lung and colon. It is likely that the major protein product of COL27A1, proa1(XXVII), is a component of the extracellular matrices of cartilage and these other tissues. Study of this collagen should yield insights into normal chondrogenesis, and provide clues to the pathogenesis of some chondrodysplasias and disorders of other tissues in which this gene is expressed (PMID: 12714037).