CNTROB Polyclonal antibody

CNTROB Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 26880-1-AP

Synonyms

Centrobin, CNTROB, LIP8, LYST interacting protein 8, PP1221

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Validation Data Gallery

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  WB analysis of mouse brain using 26880-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26880-1-AP (CNTROB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 26880-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26880-1-AP (CNTROB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 26880-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 26880-1-AP (CNTROB antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 4 publications below

Product Information

The immunogen of 26880-1-AP is CNTROB Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNTROB fusion protein Ag25454
• Full Name: centrobin, centrosomal BRCA2 interacting protein
• Calculated Molecular Weight: 903 aa, 101 kDa
• Observed Molecular Weight: 60-70 kDa, 100-130 kDa
• GenBank Accession Number: BC021134
• Gene Symbol: CNTROB
• Gene ID (NCBI): 116840
• RRID: AB_2880667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB,IF,	J Cell Biol; The central scaffold protein CEP350 coordinates centriole length, stability, and maturation; Authors - Onur Rojhat Karasu
human	IF	Elife; Global cellular response to chemical perturbation of PLK4 activity and abnormal centrosome number.; Authors - Johnny M Tkach
human	IF	Mol Biol Cell; The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis.; Authors - Alexander J Stemm-Wolf
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu