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Clusterin Monoclonal antibody

Clusterin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F12G10

Cat no : 66109-1-Ig

Synonyms

Apo-J, APOJ, Apo J, Aging-associated gene 4 protein, AAG4

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Clusterin Monoclonal antibody (66109-1-Ig)

    WB analysis of human plasma using 66109-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66109-1-Ig (Clusterin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human liver using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human liver using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human breast cancer using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human prostate cancer using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human prostate cancer using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human breast cancer using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inhuman plasma
Positive IHC detected inhuman liver tissue, human breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66109-1-Ig targets Clusterin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Clusterin fusion protein Ag19236
Full Name clusterin
Calculated Molecular Weight 449 aa, 53 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC010514
Gene Symbol CLU
Gene ID (NCBI) 1191
RRIDAB_2881508
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

Protocols

Product Specific Protocols
WB protocol for Clusterin antibody 66109-1-IgDownload protocol
IHC protocol for Clusterin antibody 66109-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols