CLPP Polyclonal antibody

• Featured Product
• KD/KO Validated

CLPP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

45

Reviews (2)

Cat no : 15698-1-AP

Synonyms

Endopeptidase Clp, EC:3.4.21.92, ATP-dependent Clp protease proteolytic subunit, mitochondrial

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Validation Data Gallery

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  WB analysis using 15698-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15698-1-AP (CLPP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 15698-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15698-1-AP (CLPP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15698-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15698-1-AP (CLPP Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 15698-1-AP

  IP result of anti-CLPP (IP:15698-1-AP, 3ug; Detection:15698-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human liver cancer using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15698-1-AP (CLPP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15698-1-AP (CLPP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15698-1-AP (CLPP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15698-1-AP (CLPP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15698-1-AP

  Immunofluorescent analysis of Hela cells, using CLPP antibody 15698-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 15698-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15698-1-AP (CLPP antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human placenta tissue, rat heart tissue, K-562 cells, mouse heart tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human liver cancer tissue, human kidney tissue, human testis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 41 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

15698-1-AP targets CLPP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, zebrafish, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLPP fusion protein Ag8325
• Full Name: ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 26 kDa, 30 kDa
• GenBank Accession Number: BC002956
• Gene Symbol: CLPP
• Gene ID (NCBI): 8192
• RRID: AB_2245115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.

Publications

Species	Application	Title
	WB	Cell Metab; Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions.; Authors - Saara Forsström
mouse	WB	Cell Metab; mTORC1 Regulates Mitochondrial Integrated Stress Response and Mitochondrial Myopathy Progression.; Authors - Nahid A Khan
mouse	WB	Cell Metab; Proteolytic cleavage of opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation.; Authors - Prashant Mishra
mouse	WB	Sci Adv; Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.; Authors - Qiqi Guo
mouse	WB	Nat Commun; Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.; Authors - Zhisheng Xu
mouse	WB	Nat Commun; Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells.; Authors - Gustavo Della-Flora Nunes

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anh (Verified Customer) (07-15-2021)

very good, very clear bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: heart cells

FH

Mariusz (Verified Customer) (02-06-2019)

Excellent antibody bot for Western blot (1:2000) dilution and immunofluorescence (1:200). Single band on Western, and clean signal reflecting mitochondrial matrix in IF.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:2000 (Western), 1:200 (IF)
• Cell Tissue Type: HeLa and HCt116