CLOCK Polyclonal antibody

CLOCK Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30680-1-AP

Synonyms

bHLHe8, CLOCK, clock homolog (mouse), hCLOCK, KAT13D, KIAA0334

---

Validation Data Gallery

• 

  IHC staining of mouse liver using 30680-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30680-1-AP (CLOCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 30680-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30680-1-AP (CLOCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 30680-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLOCK antibody (30680-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Tested Applications

• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30680-1-AP targets CLOCK in IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLOCK fusion protein Ag33370
• Full Name: clock homolog (mouse)
• Calculated Molecular Weight: 846 aa, 95 kDa
• GenBank Accession Number: BC041878
• Gene Symbol: CLOCK
• Gene ID (NCBI): 9575
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. western blot. Our result is similar to the result of reseach paper (PMID: 30683868).