• Overview
  • Tested Applications
  • Product Information
  • Publications (5)
  • Protocols

CLIP1 Polyclonal antibody

CLIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 23839-1-AP

Synonyms

RSN, Reed-Sternberg intermediate filament-associated protein, Cytoplasmic linker protein 170 alpha-2, CYLN1, CLIP-170



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue, U-937 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23839-1-AP targets CLIP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLIP1 fusion protein Ag20811
Full Name CAP-GLY domain containing linker protein 1
Calculated Molecular Weight 1438 aa, 162 kDa
Observed Molecular Weight 160-170 kDa, 135 kDa
GenBank Accession NumberBC126305
Gene Symbol CLIP1
Gene ID (NCBI) 6249
RRIDAB_2879334
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CLIP1 antibody 23839-1-APDownload protocol
IF protocol for CLIP1 antibody 23839-1-APDownload protocol
IP protocol for CLIP1 antibody 23839-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

  • Journal Impact Factor
  • Most recent
SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice.

Authors - Jingjing Zhang
humanWB

Cell Death Dis

Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration

Authors - Yunfeng Hu
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu
humanWB

Ecotoxicol Environ Saf

Exosomal circCLIP1 regulates PM2.5-induced airway obstruction via targeting SEPT10 in vitro

Authors - Huanhuan Zhu
rat,humanWB,ELISA

Exp Mol Med

Hypothermic oxygenated perfusion inhibits CLIP1-mediated TIRAP ubiquitination via TFPI2 to reduce ischemia‒reperfusion injury of the fatty liver

Authors - Pengpeng Yue