CLCA1 Recombinant antibody

CLCA1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240941G4

Cat no : 83829-7-RR

Synonyms

Calcium-activated chloride channel protein 1, Calcium-activated chloride channel family member 1, CaCC-1, CACC1, CACC

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Validation Data Gallery

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  WB analysis using 83829-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83829-7-RR (CLCA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83829-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83829-7-RR against Human CLCA1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83829-7-RR is CLCA1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CLCA1 fusion protein Ag18176
• Full Name: chloride channel accessory 1
• Calculated Molecular Weight: 914 aa, 100 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC156805
• Gene Symbol: CLCA1
• Gene ID (NCBI): 1179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calcium-activated chloride channel regulator 1(CLCA1) is a secreted protein, and it has an N-terminal zinc-dependent metallohydrolase domain with a conserved HExxE catalytic motif similar to that found in matrix metalloproteases (MMPs), and "a Disintegrin and metalloproteases" (ADAMs). The only known substrate for CLCA1 is CLCA1 itself as it undergoes intracellular autocatalytic cleavage resulting in two cleavage products which are both secreted. Since a previous study suggested that a band around 75 kDa, corresponding to the N-terminal autocatalytic cleavage product, was observed in mucus from both human and WT mice. In addition, an additional band at 50 kDa was identified in human and WT mouse mucus, suggesting that CLCA1 can be further processed. However, no band corresponding to the full-length un-cleaved CLCA1 could be detected in the human samples, and only a very faint band was observed in WT mouse mucus, indicating that most of the CLCA1 that is secreted into the mucus is fully cleaved in vivo. (PMID: 29885864)