CKAP4 Polyclonal antibody
CKAP4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 16686-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, rat kidney tissue, HepG2 cells, HeLa cells, NIH/3T3 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 21 publications below |
IHC | See 2 publications below |
IF | See 11 publications below |
IP | See 4 publications below |
CoIP | See 1 publications below |
Product Information
16686-1-AP targets CKAP4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CKAP4 fusion protein Ag10019 |
Full Name | cytoskeleton-associated protein 4 |
Calculated molecular weight | 602 aa, 66 kDa |
Observed molecular weight | 63 kDa |
GenBank accession number | BC082972 |
Gene symbol | CKAP4 |
Gene ID (NCBI) | 10970 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CKAP4, also named as Climp-63, is a 63 kDa cytoskeleton-linking membrane protein. It is the partner of triadin. CKAP4 is responsible for this association of triads and microtubules.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CKAP4 antibody 16686-1-AP | Download protocol |
IHC protocol for CKAP4 antibody 16686-1-AP | Download protocol |
IF protocol for CKAP4 antibody 16686-1-AP | Download protocol |
IP protocol for CKAP4 antibody 16686-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex | ||
Mol Cell Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex. | ||
EMBO J ER-to-lysosome-associated degradation of proteasome-resistant ATZ polymers occurs via receptor-mediated vesicular transport. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH WEI (Verified Customer) (06-28-2020) | Single strong band at correct size.
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