CKAP4 Polyclonal antibody

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  WB analysis using 16686-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16686-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 16686-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16686-1-AP

  IP result of anti-CKAP4 (IP:16686-1-AP, 4ug; Detection:16686-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human kidney using 16686-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16686-1-AP (CKAP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16686-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 16686-1-AP (CKAP4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16686-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 16686-1-AP (CKAP4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16686-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16686-1-AP (CKAP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16686-1-AP

  Immunofluorescent analysis of HepG2 cells, using CKAP4 antibody 16686-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 16686-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP4 antibody (16686-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 16686-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CKAP4 antibody (16686-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

CKAP4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16686-1-AP

Publications(48)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

p63, ERGIC 63, Climp-63, CLIMP 63, 63 kDa membrane protein

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Tested Applications

• Positive WB detected in: A431 cells, rat kidney tissue, HeLa cells, HepG2 cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 30 publications below
• IHC: See 3 publications below
• IF: See 19 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below

Product Information

16686-1-AP targets CKAP4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CKAP4 fusion protein Ag10019
• Full Name: cytoskeleton-associated protein 4
• Calculated Molecular Weight: 602 aa, 66 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC082972
• Gene Symbol: CKAP4
• Gene ID (NCBI): 10970
• RRID: AB_2276275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q07065
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CKAP4, also named as Climp-63, is a 63 kDa cytoskeleton-linking membrane protein. It is the partner of triadin. CKAP4 is responsible for this association of triads and microtubules.

Publications

Species	Application	Title
mouse	WB	Nature; Regulation of endoplasmic reticulum turnover by selective autophagy.; Authors - Aliaksandr Khaminets
rat	WB	Cell Metab; Palmitoylation couples insulin hypersecretion with β cell failure in diabetes; Authors - Guifang Dong
human	WB	Nat Cell Biol; Combinatorial selective ER-phagy remodels the ER during neurogenesis; Authors - Melissa J Hoyer
human	WB	Nat Commun; AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity; Authors - Linliang Zhang
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex; Authors - Michael L van de Weijer
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.; Authors - Michael L van de Weijer

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (06-28-2020)

Single strong band at correct size.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse Heart