CIDEA Polyclonal antibody

CIDEA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 13170-1-AP

Synonyms

Cell death activator CIDE A, CIDE A, CIDEA

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Validation Data Gallery

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  WB analysis of mouse heart using 13170-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13170-1-AP (CIDEA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13170-1-AP

  Mouse heart tissue was subjected to SDS PAGE followed by western blot with 13170-1-AP (CIDEA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brown adipose using 13170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 13170-1-AP (CIDEA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 13170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 13170-1-AP (CIDEA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: mouse brown adipose tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

13170-1-AP targets CIDEA in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CIDEA fusion protein Ag3824
• Full Name: cell death-inducing DFFA-like effector a
• Calculated Molecular Weight: 253 aa, 28 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC031896
• Gene Symbol: CIDEA
• Gene ID (NCBI): 1149
• RRID: AB_10642957
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell death-inducing DNA fragmentation factor (DFFA)-like effector (CIDE) family proteins, including Cidea, Cideb, and Fsp27 (Cidec), are lipid droplet (LD)-associated proteins playing important roles in LD fusion, lipid secretion, and very-low-density-lipoprotein (VLDL) maturation. Cidea is highly enriched in adult brown adipose tissue (BAT), and its deficiency results in the accumulation of smaller LDs in brown adipocytes, improved INS sensitivity, and resistance to diet-induced obesity. The MW of this protein is 25 kDa, and this antibody specially recognises the 25 kDa protein.

Publications

Species	Application	Title
mouse	WB	Nature; CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization; Authors - Kevin Qian
mouse	IF	Nat Commun; Dynamic enlargement and mobilization of lipid droplets in pluripotent cells coordinate morphogenesis during mouse peri-implantation development.; Authors - King Hang Tommy Mau
mouse	WB	EMBO J; Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues.; Authors - Yingjiang Xu
mouse	WB	Diabetes; Fat-Specific Knockout of Mecp2 Upregulates Slpi to Reduce Obesity by Enhancing Browning.; Authors - Chengyu Liu
mouse	WB,IHC	J Agric Food Chem; Polysaccharide from Ziyang Selenium-Enriched Green Tea Prevents Obesity and Promotes Adipose Thermogenesis via Modulating the Gut Microbiota; Authors - Donglu Li
mouse	WB,IHC	Food Funct; Artemisia sphaerocephala Krasch polysaccharide promotes adipose thermogenesis and decreases obesity by shaping the gut microbiota; Authors - Xiaoqian Zeng