CIB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CIB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 11823-1-AP

Synonyms

DNA PKcs interacting protein, CIBP, CIB, Calmyrin, Calcium and integrin-binding protein 1

---

Validation Data Gallery

• 

  WB analysis of human liver using 11823-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11823-1-AP (CIB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 11823-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11823-1-AP (CIB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11823-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11823-1-AP (CIB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 11823-1-AP

  IP result of anti-CIB1 (IP:11823-1-AP, 4ug; Detection:11823-1-AP 1:300) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 11823-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11823-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11823-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat testis using 11823-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat testis using 11823-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 11823-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CIB1 antibody (11823-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: human liver tissue, HepG2 cells, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human breast cancer tissue, human testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

11823-1-AP targets CIB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CIB1 fusion protein Ag2391
• Full Name: calcium and integrin binding 1 (calmyrin)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC000846
• Gene Symbol: CIB1
• Gene ID (NCBI): 10519
• RRID: AB_2079679
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CIB1(Calcium and integrin-binding protein 1) is also named as CIB, KIP, PRKDCIP. It is an EF-hand-containing protein that binds multiple effector proteins, including the platelet αIIbβ3 integrin and several serine/threonine kinases and potentially modulates their function. It regulates platelet aggregation in hemostasis through a specific interaction with the alphaIIb cytoplasmic domain of platelet integrin alphaIIbbeta3.

Publications

Species	Application	Title
human	IP	Cell Death Differ; CHIP-mediated CIB1 ubiquitination regulated epithelial-mesenchymal transition and tumor metastasis in lung adenocarcinoma.; Authors - Yuanqi Liu
human	WB,IF	PLoS Pathog; CIB1 Synergizes with EphrinA2 to Regulate Kaposi's Sarcoma-Associated Herpesvirus Macropinocytic Entry in Human Microvascular Dermal Endothelial Cells.; Authors - Chirosree Bandyopadhyay ; KD Validated
mouse	WB	FASEB J; Identification of calcium and integrin-binding protein 1 as a novel regulator of production of amyloid β peptide using CRISPR/Cas9-based screening system.; Authors - Yung Wen Chiu ; KO Validated
rat	WB	Sci Rep; PDI regulates seizure activity via NMDA receptor redox in rats.; Authors - Ji Yang Kim
human	WB, IF	J Virol; p130Cas Scaffolds the Signalosome to Direct Adaptor-Effector Crosstalk During Kaposi's Sarcoma-Associated Herpesvirus Trafficking in Human Microvascular Dermal Endothelial Cells.; Authors - Chirosree Bandyopadhyay ; KD Validated
mouse	WB	Front Mol Neurosci; Expression of genes encoding the calcium signalosome in cellular and transgenic models of Huntington's disease.; Authors - Magdalena Czeredys