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  • KD/KO Validated

CIB1 Polyclonal antibody

CIB1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 11823-1-AP


Calmyrin, CIB, CIB1, CIBP, DNA PKcs interacting protein, Kinase interacting protein, KIP, KIP1, PRKDCIP, SIP2 28, SNK interacting protein 2 28

Tested Applications

Positive WB detected inhuman liver tissue, HepG2 cells, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue, human testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11823-1-AP targets CIB1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CIB1 fusion protein Ag2391
Full Name calcium and integrin binding 1 (calmyrin)
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC000846
Gene Symbol CIB1
Gene ID (NCBI) 10519
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CIB1(Calcium and integrin-binding protein 1) is also named as CIB, KIP, PRKDCIP. It is an EF-hand-containing protein that binds multiple effector proteins, including the platelet αIIbβ3 integrin and several serine/threonine kinases and potentially modulates their function. It regulates platelet aggregation in hemostasis through a specific interaction with the alphaIIb cytoplasmic domain of platelet integrin alphaIIbbeta3.


Product Specific Protocols
WB protocol for CIB1 antibody 11823-1-APDownload protocol
IHC protocol for CIB1 antibody 11823-1-APDownload protocol
IF protocol for CIB1 antibody 11823-1-APDownload protocol
IP protocol for CIB1 antibody 11823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Death Differ

CHIP-mediated CIB1 ubiquitination regulated epithelial-mesenchymal transition and tumor metastasis in lung adenocarcinoma.

Authors - Yuanqi Liu

PLoS Pathog

CIB1 Synergizes with EphrinA2 to Regulate Kaposi's Sarcoma-Associated Herpesvirus Macropinocytic Entry in Human Microvascular Dermal Endothelial Cells.

Authors - Chirosree Bandyopadhyay
  • KD Validated


Identification of calcium and integrin-binding protein 1 as a novel regulator of production of amyloid β peptide using CRISPR/Cas9-based screening system.

Authors - Yung Wen Chiu
  • KO Validated

Sci Rep

PDI regulates seizure activity via NMDA receptor redox in rats.

Authors - Ji Yang Kim
humanWB, IF

J Virol

p130Cas Scaffolds the Signalosome to Direct Adaptor-Effector Crosstalk During Kaposi's Sarcoma-Associated Herpesvirus Trafficking in Human Microvascular Dermal Endothelial Cells.

Authors - Chirosree Bandyopadhyay
  • KD Validated

Front Mol Neurosci

Expression of genes encoding the calcium signalosome in cellular and transgenic models of Huntington's disease.

Authors - Magdalena Czeredys