CHRAC1 Polyclonal antibody

CHRAC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12126-2-AP

Synonyms

CHARC1, CHARC15, CHRAC 1, CHRAC 15, CHRAC1, CHRAC15, HuCHRAC15, YCL1

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Validation Data Gallery

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  WB analysis using 12126-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12126-2-AP (CHRAC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12126-2-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12126-2-AP (CHRAC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12126-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12126-2-AP (CHRAC1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, L02 cells, HeLa cells, HepG2 cells, K-562 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12126-2-AP targets CHRAC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHRAC1 fusion protein Ag2770
• Full Name: chromatin accessibility complex 1
• Calculated Molecular Weight: 131 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC015891
• Gene Symbol: CHRAC1
• Gene ID (NCBI): 54108
• RRID: AB_2291729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.