Cofilin Polyclonal antibody

• Featured Product
• KD/KO Validated

Cofilin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 10960-1-AP

Synonyms

CFL1, 18 kDa phosphoprotein, Cofilin-1, Cofilin1, Cofilin, non-muscle isoform

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Validation Data Gallery

• 

  WB analysis using 10960-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10960-1-AP (Cofilin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10960-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10960-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10960-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skin using 10960-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10960-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (Cofilin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10960-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10960-1-AP (Cofilin antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human lung cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 10960-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Cofilin antibody (10960-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 10960-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 10960-1-AP (Cofilin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, NIH/3T3 cells, human skin tissue, human brain tissue, mouse brain tissue, rat brain tissue, NCI-H1299 cells
• Positive IHC detected in: human colon tissue, human cervical cancer tissue, human heart tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:600-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 22 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

10960-1-AP targets Cofilin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cofilin fusion protein Ag1399
• Full Name: cofilin 1 (non-muscle)
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18-22 kDa
• GenBank Accession Number: BC012318
• Gene Symbol: Cofilin
• Gene ID (NCBI): 1072
• RRID: AB_2291830
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation.Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

Publications

Species	Application	Title
	WB,IF,IP	Nat Commun; N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization; Authors - Weiji Weng
human	WB,IF	Cell Res; NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.; Authors - Cheng Zhang ; KD Validated
human	WB	Int J Mol Sci; Protein Kinase C Activation Drives a Differentiation Program in an Oligodendroglial Precursor Model through the Modulation of Specific Biological Networks.; Authors - Marina Damato
mouse	WB	Commun Biol; RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling; Authors - Haixia Zhu
chicken	WB	Microbiol Spectr; Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.; Authors - Beibei Zhang
human	IHC	Oncotarget; A three-protein signature and clinical outcome in esophageal squamous cell carcinoma.; Authors - Hui-Hui Cao

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