CEBPA Polyclonal antibody

• Featured Product
• KD/KO Validated

CEBPA Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

63

Cat no : 18311-1-AP

Synonyms

C/EBP alpha, C/EBPα, CEBP, CEBPA

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Validation Data Gallery

• 

  WB analysis of L02 using 18311-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 18311-1-AP (CEBPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 18311-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 18311-1-AP (CEBPA antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 18311-1-AP

  IP result of anti-CEBPA (IP:18311-1-AP, 4ug; Detection:18311-1-AP 1:500) with L02 cells lysate 1800ug.

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Tested Applications

• Positive WB detected in: L02 cells, human liver tissue
• Positive IP detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 56 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• ChIP: See 7 publications below

Product Information

18311-1-AP targets CEBPA in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, hamster, sheep, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: CCAAT/enhancer binding protein (C/EBP), alpha
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC160133
• Gene Symbol: CEBPA
• Gene ID (NCBI): 1050
• RRID: AB_2077892
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEBPA and its isoforms play important roles in lineage determination and gene activation in a variety of cell types by activating transcription from lineage-specific promoters. CEBPA is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. In hematopoiesis, C/EBPa is a key factor in driving the development of myeloid cells interacting with a variety of factors, including c-Myc, PU.1, and microRNAs. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin which plays an important role in body weight homeostasis. CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Several pathways have been implicated as the means by which CEBPA mediates cell cycle arrest and proliferation, including p21, cyclin-dependent kinases and the E2F complex via c-Myc. The calcualted molecular weight of CEBPA is 38 kDa, but modified CEBPA is about 42 kDa (PMID: 19623175).

Publications

Species	Application	Title
human	WB	Cell Metab; CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.; Authors - Qidong Li ; KD Validated
mouse	WB	Exp Mol Med; Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury; Authors - Zhenjun Yu
	ChIP	J Clin Invest; Dysregulation of PI3K and hippo signaling pathways synergistically induces chronic pancreatitis via Ctgf upregulation.; Authors - Takeshi Tamura
mouse	ChIP	Bone Res; mTORC1 induces plasma membrane depolarization and promotes preosteoblast senescence by regulating the sodium channel Scn1a.; Authors - Ajuan Chen
human	WB	Theranostics; PIWIL3/OIP5-AS1/miR-367-3p/CEBPA feedback loop regulates the biological behavior of glioma cells.; Authors - Xiaobai Liu ; KD Validated
human	WB	Leukemia; Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells.; Authors - Ladan Kobari

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