CDX1 Polyclonal antibody

CDX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21655-1-AP

Synonyms

Homeobox protein CDX-1, Homeobox protein CDX 1, Caudal-type homeobox protein 1, Caudal type homeobox protein 1, Caudal type homeobox 1

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Validation Data Gallery

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  WB analysis using 21655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 21655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 21655-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse stomach using 21655-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 21655-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 21655-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 21655-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Caco-2 using 21655-1-AP

  IP result of anti-CDX1 (IP:21655-1-AP, 4ug; Detection:21655-1-AP 1:1000) with Caco-2 cells lysate 1400 ug.

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  IHC staining of human colon cancer using 21655-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21655-1-AP (CDX1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 21655-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX1 antibody (21655-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: Caco-2 cells, mouse small intestine tissue, mouse stomach tissue, mouse colon tissue, rat colon tissue
• Positive IP detected in: Caco-2 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21655-1-AP targets CDX1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDX1 fusion protein Ag16376
• Full Name: caudal type homeobox 1
• Calculated Molecular Weight: 265 aa, 28 kDa
• Observed Molecular Weight: 28 kDa, 35 kDa
• GenBank Accession Number: BC096252
• Gene Symbol: CDX1
• Gene ID (NCBI): 1044
• RRID: AB_2878901
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.