CDC42EP4 Polyclonal antibody

CDC42EP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19262-1-AP

Synonyms

CEP4, Cdc42 effector protein 4, BORG4, Binder of Rho GTPases 4

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Validation Data Gallery

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  WB analysis of K-562 using 19262-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19262-1-AP (CDC42EP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 19262-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19262-1-AP (CDC42EP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 19262-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19262-1-AP (CDC42EP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 19262-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 19262-1-AP (CDC42EP4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 19262-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 19262-1-AP (CDC42EP4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, A549 cells, mouse lung tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19262-1-AP targets CDC42EP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC42EP4 fusion protein Ag13594
• Full Name: CDC42 effector protein (Rho GTPase binding) 4
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: BC010451
• Gene Symbol: CDC42EP4
• Gene ID (NCBI): 23580
• RRID: AB_10598005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC42EP4, or Cell division cycle 42 effector protein 4, is a member of the CDC42-binding protein family. This protein interacts with Rho family GTPases and plays a role in regulating the organization of the actin cytoskeleton. It has been shown to bind both CDC42 and TC10 GTPases in a GTP-dependent manner. Overexpression of CDC42EP4 in fibroblasts can induce pseudopodia formation, suggesting its involvement in actin filament assembly and cell shape control. CDC42EP4 is also implicated in cancer progression, particularly in prostate cancer. Furthermore, CDC42EP4 has been implicated in the regulation of glutamate transporters, which are crucial for maintaining glutamate homeostasis in the nervous system. It has been suggested that CDC42EP4 may interact with septins, a family of polymerizing GTPases that constitute the membrane skeleton, and this interaction could potentially influence the localization and activity of glutamate transporters.