CDC25C Polyclonal antibody

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  WB analysis using 16485-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16485-1-AP (CDC25C antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16485-1-AP

  WB result of CDC25C antibody (16485-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDC25C transfected HeLa cells.

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  WB analysis of HeLa using 16485-1-AP

  Non-treated HeLa and nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 16485-1-AP (CDC25C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis of Raji using 16485-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 16485-1-AP (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 16485-1-AP

  IP result of anti-CDC25C (IP:16485-1-AP, 4ug; Detection:16485-1-AP 1:1000) with K-562 cells lysate 3600ug.

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  IHC staining of human colon cancer using 16485-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16485-1-AP (CDC25C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16485-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16485-1-AP (CDC25C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16485-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16485-1-AP (CDC25C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16485-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16485-1-AP (CDC25C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

CDC25C Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 16485-1-AP

Publications(32)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

CDC25, CDC25C, M phase inducer phosphatase 3

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Tested Applications

• Positive WB detected in: HEK-293 cells, Raji cells, HeLa cells, HepG2 cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 32 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Product Information

16485-1-AP targets CDC25C in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC25C fusion protein Ag9607
• Full Name: cell division cycle 25 homolog C (S. pombe)
• Calculated Molecular Weight: 473 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC019089
• Gene Symbol: CDC25C
• Gene ID (NCBI): 995
• RRID: AB_2291330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P30307
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C(cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells(PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop(PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa(PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.

Publications

Species	Application	Title
human	WB,IHC	Nat Commun; A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.; Authors - Xiaojie Xu
human	WB	Phytother Res; Activation of endoplasmic reticulum stress by harmine suppresses the growth of esophageal squamous cell carcinoma; Authors - Ronghui Fan
human	WB	Cell Death Dis; PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT.; Authors - Wen-Kai Shi
human	WB,IHC	J Ethnopharmacol; Kaempferol from Alpinia officinarum Hance induces G2/M cell cycle arrest in hepatocellular carcinoma cells by regulating the ATM/CHEK2/KNL1 pathway; Authors - Xiaoliang Li
human	WB	Cell Cycle; PMID: 31599189; Authors - Yonglong Zhang
human	WB	Oncotarget; Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.; Authors - Yi Huo