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CDC25C Polyclonal antibody

CDC25C Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB,IP,IHC,FC,ELISA

Conjugate

Unconjugated

Cat no : 16485-1-AP

Synonyms

CDC25, CDC25C, M phase inducer phosphatase 3



Tested Applications

Positive WB detected inHEK-293 cells, Raji cells, HeLa cells, HepG2 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16485-1-AP targets CDC25C in WB,IP,IHC,FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC25C fusion protein Ag9607
Full Name cell division cycle 25 homolog C (S. pombe)
Calculated Molecular Weight 473 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC019089
Gene Symbol CDC25C
Gene ID (NCBI) 995
RRIDAB_2291330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C(cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells(PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop(PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa(PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.

Protocols

Product Specific Protocols
WB protocol for CDC25C antibody 16485-1-APDownload protocol
IHC protocol for CDC25C antibody 16485-1-APDownload protocol
IP protocol for CDC25C antibody 16485-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Nat Commun

A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.

Authors - Xiaojie Xu
humanWB

Phytother Res

Activation of endoplasmic reticulum stress by harmine suppresses the growth of esophageal squamous cell carcinoma

Authors - Ronghui Fan
humanWB

Cell Death Dis

PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT.

Authors - Wen-Kai Shi
humanWB

Cell Cycle

PMID: 31599189

Authors - Yonglong Zhang
humanWB

Oncotarget

Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.

Authors - Yi Huo
humanWB

Oncol Res

Therapeutic Targeting PLK1 by ON-01910.Na Is Effective in Local Treatment of Retinoblastoma

Authors - Huan Ma