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CDC25C Polyclonal antibody

CDC25C Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 16485-1-AP


CDC25, CDC25C, M phase inducer phosphatase 3

Tested Applications

Positive WB detected inHEK-293 cells, Raji cells, HeLa cells, HepG2 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16485-1-AP targets CDC25C in WB,IP,IHC,FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC25C fusion protein Ag9607
Full Name cell division cycle 25 homolog C (S. pombe)
Calculated Molecular Weight 473 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC019089
Gene Symbol CDC25C
Gene ID (NCBI) 995
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C(cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells(PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop(PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa(PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.


Product Specific Protocols
WB protocol for CDC25C antibody 16485-1-APDownload protocol
IHC protocol for CDC25C antibody 16485-1-APDownload protocol
IP protocol for CDC25C antibody 16485-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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