CD97 Polyclonal antibody, PBS Only

CD97 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13071-1-PBS

Synonyms

CD97 molecule, CD97 antigen, Adhesion G protein-coupled receptor E5 subunit beta, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5

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Validation Data Gallery

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  WB analysis using 13071-1-AP (same clone as 13071-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13071-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 13071-1-AP (same clone as 13071-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13071-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 13071-1-AP (same clone as 13071-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13071-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 13071-1-AP (same clone as 13071-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13071-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 13071-1-AP (same clone as 13071-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13071-1-AP (CD97 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13071-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 13071-1-AP (same clone as 13071-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13071-1-AP (CD97 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13071-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 13071-1-AP (same clone as 13071-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 13071-1-AP (CD97 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13071-1-PBS in a different storage buffer formulation.

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Product Information

13071-1-PBS targets CD97 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD97 fusion protein Ag3745
• Full Name: CD97 molecule
• Calculated Molecular Weight: 722 aa, 79 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC026690
• Gene Symbol: CD97
• Gene ID (NCBI): 976
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).